To those compounds that belong to phenolics, most of which express relevant in vitro and in vivo antioxidant activities and ascorbic acid (Skrovankova et al. Strawberries are characterized by a high amount of antioxidant molecules, for example, they have a high antioxidant capacity that is closely linked to the currently available efficient oxygen radical scavengers (Biswas et al. The phenolic compounds present in strawberries influence their quality, contributing to sensorial and organoleptic attributes, as well as to health properties (Guine et al. The major class of strawberry polyphenols is flavonoids, mainly anthocyanins, the most quantitatively important phenolic compounds present in strawberries in the form of pelargonidin and cyanidin derivates (Giampieri et al. They also have some indirect antimicrobial, anti-allergy, and anti-hypertensive properties, as well as the ability to inhibit the activity of some physiological enzymes and receptors, which helps to prevent diseases caused by oxidative stress (Afrin et al. Strawberry phenolics are best known, of course, for their antioxidant and anti-inflammatory properties recently researchers found that some phenolics compounds can act as anti-COVID-19 drug agents due to their potential inhibitory activity on SARS-CoV-2 (Bhushan et al.

The primary phytochemicals in strawberries are phenolic compounds, a sizable and diverse group of biologically active non-nutrients that exhibit a wide range of non-essential biological roles in plants and biologically important potential for people (Zitouni et al. Strawberry consumption ranks among the highest dietary sources of phytochemical antioxidants such vitamin C, carotenoids, and phenolic compounds (Šic Žlabur et al. One of the most widely consumed berry fruits, strawberries ( Fragaria x ananassa Duch.) are mostly consumed as fresh fruit but are also used as a component in processed foods globally (Markovinović et al.

Additionally, the potential health benefits of these species, primarily attributable to their phytochemical composition and antioxidant activity, have stimulated interest in research on fruit species high in polyphenols (Rudrapal et al. Numerous epidemiological studies conducted over the past few decades have demonstrated that consuming a fruit-rich diet can lower the chance of developing a number of serious chronic diseases that affect humans (Li et al. Berry fruits play a significant role in human nutrition and provide numerous health benefits because of their high nutrient content, which includes vitamins, minerals, and bioactive components such polyphenolic phytochemicals (Olas 2018 Golovinskaia and Wang 2021 Cosme et al. Additionally, it has been suggested that they could be useful for the prevention of several chronic and neurodegenerative disorders (Zehiroglu and Ozturk Sarikaya 2019 Tulli et al. These substances possess many advantages including anti-aging and anti-inflammatory. The majority of the most significant antioxidants in the diet, such as polyphenolic chemicals and ascorbic acid, may be found naturally in fruits and vegetables (Zhang et al.

Furthermore, these results indicate that strawberry fruits ( Fragaria x ananassa Duch.) can be a natural food colorant in dietary applications with potential health benefits. The present model can contribute to finding a cheap way of delivering natural antioxidants in the food, cosmeceutical, and pharmaceutical industries. The results indicated that optimizing extraction conditions is critical for quantifying antioxidant phenolic compounds. The maximum TPC and TFC values were found as 18.78 ± 0.22 mg of gallic acid equivalent (GAE/g) and 10.52 ± 0.35 mg of catechin equivalents (CE/g) under optimum extraction conditions. The optimal extraction conditions for both responses were determined to be time of 17.5 min, temperature 52.5 ☌, and liquid/solid ratio of 30:1. In the study, extracts obtained with acetone indicated the highest total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity.

Box-Behnken Design was used to optimize extraction conditions, including extraction time (t), temperature (☌), and liquid/solid (L/S) ratio. Extractions were carried out using solvents with different polarities (water, methanol, ethanol, acetonitrile, and acetone). The subject of this study is to determine the best solvent and optimum extraction conditions for the extraction of maximum antioxidant phenolic compounds and antioxidant activity from strawberry fruits ( Fragaria x ananassa Duch.).

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